Difference between revisions of "Part:BBa F2620"
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|width='80px'|[[Input Swing|''Input Swing:'']] | |width='80px'|[[Input Swing|''Input Swing:'']] | ||
| − | |width='260px'|'''0.1 | + | |width='260px'|'''0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous''' |
|width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | |width='130px' align='left'|[[Resonse time|''L/H Response time:'']] | ||
| − | |width='210px' align='left'|'''25''' | + | |width='210px' align='left'|'''25 mins''' |
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|[[Output Swing|''Output Swing:'']] | |[[Output Swing|''Output Swing:'']] | ||
Revision as of 21:05, 5 October 2006
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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
| Characteristics | |||
| Input Swing: | 0.1 - 1000 nM 3OC6HSL, exogenous | L/H Response time: | 25 mins |
| Output Swing: | 21±3 - 590±9 GFP molecules cfu-1 s-1 | Genetic Stability: | >92/74 generations (Low/High Input) |
| Switch Point: | 1E-8 M 3OC6HSL, exogenous | Performance Stability: | >92/74 generations (Low/High Input) |
| Transfer Function |
Specificity |
| Response time |
Stability |
| Demand | |||
| Translation Demand: (100 nM AHL input) |
141600 charged tRNA cfu-1 s-1 9440 ribosomes sequestered cfu-1 |
Growth Rate: | 54/59 min doubling time (Low/High Input) |
Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing BBa_C0040.