Difference between revisions of "Part:BBa F2620"

Revision as of 21:05, 5 October 2006

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing:	0.1 - 1000 nM 3OC₆HSL, exogenous	L/H Response time:	25 mins
Output Swing:	21±3 - 590±9 GFP molecules cfu^-1 s^-1	Genetic Stability:	>92/74 generations (Low/High Input)
Switch Point:	1E-8 M 3OC₆HSL, exogenous	Performance Stability:	>92/74 generations (Low/High Input)

Transfer Function	Specificity
Response time	Stability

Demand
Translation Demand: (100 nM AHL input)	141600 charged tRNA cfu^-1 s^-1 9440 ribosomes sequestered cfu^-1	Growth Rate:	54/59 min doubling time (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing BBa_C0040.

@@ Line 4: / Line 4: @@
 '''Description'''<br>
 A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
 </div>
 {|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
@@ Line 10: / Line 9: @@
 |-
 |width='80px'|[[Input Swing|''Input Swing:'']]
-|width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+|width='260px'|'''0.1''' - '''1000''' nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 |width='130px' align='left'|[[Resonse time|''L/H Response time:'']]
-|width='210px' align='left'|25 mins
+|width='210px' align='left'|'''25''' mins
 |-
 |[[Output Swing|''Output Swing:'']]
-|13.6 - 490.2 GFP molecules/s.cfu
+|'''21±3 - 590±9 GFP molecules cfu<sup>-1</sup> s<sup>-1</sup>'''
 |align='left'|[[Genetic Stability|''Genetic Stability:'']]
-|>92/74 generations (Low/High Input)
+|'''>92/74 generations''' (Low/High Input)
 |-
 |[[Switch Point|''Switch Point:'']]
-|8 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+|'''1E-8 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous'''
 |align='left'|[[Performance Stability|''Performance Stability:'']]
-|>92/74 generations (Low/High Input)
+|'''>92/74 generations''' (Low/High Input)
 |}
 {|align="center" cellspacing="0" cellpadding="0"
@@ Line 40: / Line 39: @@
 |width='680px'  colspan="4" valign="top"|'''Demand'''
 |-
-|width='130px' valign="top"|[[Transcription Demand|''Transcription Demand:'']]
+|width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]<br>('''100 nM''' AHL input)
-|width='210px' valign="top"|## NTP per second<br>## polymerases sequestered
+|width='215px' valign="top"| '''141600 charged tRNA cfu<sup>-1</sup> s<sup>-1</sup>'''<br>'''9440 ribosomes sequestered cfu<sup>-1</sup>'''
-|width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]
+|width='125px' valign="top"| [[Growth Rate|''Growth Rate:'']]
-|width='215px' valign="top"| # charged tRNA per second<br># ribosomes sequestered
+|width='215px' valign="top"| 54/59 min doubling time (Low/High Input)
 |}
 </div>