Difference between revisions of "Part:BBa K1379004"

Revision as of 08:39, 7 October 2014

S. pneumoniae σ^x CDS

Gene sequence of competence protein comX which produce SigmaX factor. This sigmaX is the inducer for PcelA promoter (BBa_K1379002), and Phelicase promoter (BBa_K1379003) encoding GFP. This sigmaX protein will bind to 8 base pairs of PcelA or Phelicase promoter and trigger gene expression. The sigmaX gene was cloned from E. coli NCTC 7465 strain.

Figure 1. PcelA and Phelicase promoter induced by SigmaX protein drives GFP expression. While the same construct without SigmaX protein did not give any GFP signals. Another negative control which is only protein sigmaX without PcelA or Phelicase also did not give any GFP signals. Reference promoter BBa_J23100 + GFP is used as positive control. Scale bar = 5mm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 298

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 __NOTOC__
 <partinfo>BBa_K1379004 short</partinfo>
-Gene sequence of  competence protein comX which produce SigmaX factor.
+Gene sequence of competence protein comX which produce SigmaX factor. This sigmaX is the inducer for PcelA promoter ([[Part:BBa_K1379002|BBa_K1379002]]), and Phelicase promoter ([[Part:BBa_K1379003|BBa_K1379003]]) encoding GFP. This sigmaX protein will bind to 8 base pairs of PcelA or Phelicase promoter and trigger gene expression. The sigmaX gene was cloned from E. coli NCTC 7465 strain.
+<br><br>
+<br>
+[[File:pcelAphelicase.png|500px|thumb|center|'''Figure 1. PcelA and Phelicase promoter induced by SigmaX protein drives GFP expression. While the same construct without SigmaX protein did not give any GFP signals. Another negative control which is only protein sigmaX without PcelA or Phelicase also did not give any GFP signals. Reference promoter [[Part:BBa_J23100|BBa_J23100]] + GFP is used as positive control. Scale bar = 5mm.]]
 <!-- Add more about the biology of this part here