Difference between revisions of "Part:BBa K1351025"
| Line 11: | Line 11: | ||
|- | |- | ||
|suffix with SpeI, NotI and PstI: | |suffix with SpeI, NotI and PstI: | ||
| − | |T<span style="color:red"> | + | |T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span> |
|} | |} | ||
Sites of restriction enzymes generating compatible overhangs have the same color: | Sites of restriction enzymes generating compatible overhangs have the same color: | ||
Revision as of 07:17, 7 October 2014
PcomAB, a CSP inducible promoter derived from Streptococcus pneumoniae
CSP-depending promoter of Streptococcus pneumoniae.
This part was generated with the RFC10 standard and has the following prefix and suffix:
| prefix with EcoRI, NotI and XbaI: | GAATTCGCGGCCGCTTCTAGAG |
| suffix with SpeI, NotI and PstI: | TACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]