Difference between revisions of "Part:BBa K1351025"
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CSP-depending promoter of ''Streptococcus pneumoniae''.
 
CSP-depending promoter of ''Streptococcus pneumoniae''.
  
−
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:  
+
This part was generated in a modified version of RFC10 and has the following prefix and suffix:  
  
 
{|
 
{|
−
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:  
+
|prefix with EcoRI, NotI and XbaI:  
−
|<span style="color:blue">GAATTCC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>T<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>  
+
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>  
 
|-
 
|-
 
|suffix with AgeI, SpeI, NotI and PstI:  
 
|suffix with AgeI, SpeI, NotI and PstI:  

Revision as of 07:03, 7 October 2014

PcomAB, a CSP inducible promoter derived from Streptococcus pneumoniae

CSP-depending promoter of Streptococcus pneumoniae.

This part was generated in a modified version of RFC10 and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAGAAGGAGGAGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]