Difference between revisions of "Part:BBa K1379007:Design"

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===Design Notes===
 
===Design Notes===
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N/A
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None
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===Source===
 
===Source===

Revision as of 03:42, 7 October 2014


σx Generator + PcomFA-E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359
    Illegal BsaI.rc site found at 1550


Design Notes

None

Source

- Phelicase primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute. - both comX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain
- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014 - Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013 - GFP generator (BBa_E0240) was obtained from iGEM distribution kit 2014

References