Difference between revisions of "Part:BBa K1379007:Design"

 
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===Source===
 
===Source===
  
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Both comX gene and Phelicase are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT, downstream of PCelA, is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
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- Phelicase primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute.
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- both comX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain<br>
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- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014
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- Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013
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- GFP generator (BBa_E0240) was obtained from iGEM distribution kit 2014
  
 
===References===
 
===References===

Revision as of 03:42, 7 October 2014


σx Generator + PcomFA-E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359
    Illegal BsaI.rc site found at 1550


Design Notes

N/A


Source

- Phelicase primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute. - both comX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain
- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014 - Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013 - GFP generator (BBa_E0240) was obtained from iGEM distribution kit 2014

References