Difference between revisions of "Part:BBa K1341000"
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<partinfo>BBa_K1341000 short</partinfo>
 
<partinfo>BBa_K1341000 short</partinfo>
  
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We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.
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We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.If this device(BBa_K1341000) and tetR(BBa_K1341003)&lacI(BBa_K1341002)successful  inserted in the path way by using the typically enzyme site. It will drive expression of green fluorescent protein (GFP). This characteristic will help us to screen out the possible way we want to find.PS: We build a measurement system to test the screen functional about this device in our project(BBa_K1341000) JIA YUAN LI TU.
  
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==Assembly standards==
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===model===
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This part is based on Part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  We resynthesised this part to remove three PstI restriction sites, making it compatible with BioBrick assembly standards 10 and 23.
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JIA MO XING TU
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===characteristic===
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Unfortunately, in the process of mutagenesis to create the colour change a SpeI site was introduced at site 3044. We have ordered primers to remove this site by a substitution from T->C at base 3046.  [[BBa K325259]] (planning) will be the successor to this part compatible with Assembly Standards 10 and 23. Unfortunately we did not have time to complete this before the submission deadline, therefore the part will be submitted during November 2010.  In the meantime it is possible to use this part with <html>RFC 57</html>.
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JIA TE ZHENG TU
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Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 100 ng/mL aTc and 1mM IPTG.  
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===Functional Parameters===
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This panel depicts an LTT promoter design, where the single lac operator site has been moved upstream of the
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−35 sequence. In this case, LacI is no longer able to entirely suppress transcription and an intermediate level of induction (leakiness) occurs at conditions of high aTc but no IPTG. All data is obtained 6 h after induction.
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===Functional Parameters===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1341000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1341000 SequenceAndFeatures</partinfo>

Revision as of 03:26, 7 October 2014

switch device(LTT gfp)for our bio-compass

We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.If this device(BBa_K1341000) and tetR(BBa_K1341003)&lacI(BBa_K1341002)successful inserted in the path way by using the typically enzyme site. It will drive expression of green fluorescent protein (GFP). This characteristic will help us to screen out the possible way we want to find.PS: We build a measurement system to test the screen functional about this device in our project(BBa_K1341000) JIA YUAN LI TU.

model

JIA MO XING TU

characteristic

JIA TE ZHENG TU Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 100 ng/mL aTc and 1mM IPTG. This panel depicts an LTT promoter design, where the single lac operator site has been moved upstream of the −35 sequence. In this case, LacI is no longer able to entirely suppress transcription and an intermediate level of induction (leakiness) occurs at conditions of high aTc but no IPTG. All data is obtained 6 h after induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 732