Difference between revisions of "Part:BBa K1341000"

Revision as of 01:28, 7 October 2014

switch device(LTT gfp)for our bio-compass

We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.

Usage and Biology

Model for the device(BBa_K1341000) with LTT promoter

Sequence and Features

Functional Parameters

Assembly standards

This part is based on Part BBa_K325219. We resynthesised this part to remove three PstI restriction sites, making it compatible with BioBrick assembly standards 10 and 23.

Unfortunately, in the process of mutagenesis to create the colour change a SpeI site was introduced at site 3044. We have ordered primers to remove this site by a substitution from T->C at base 3046. BBa K325259 (planning) will be the successor to this part compatible with Assembly Standards 10 and 23. Unfortunately we did not have time to complete this before the submission deadline, therefore the part will be submitted during November 2010. In the meantime it is possible to use this part with RFC 57.

Sequences