Difference between revisions of "Part:BBa F2620"
Line 1: Line 1:
 
<div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
 
<div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
−
{{Template:F2620 page header|
+
{{Template:F2620 page header}}
−
|Last Update=15th Sept. 2006
+
−
}}
+
 
<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 
<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 
'''Description'''<br>
 
'''Description'''<br>
Line 9: Line 7:
 
</div>
 
</div>
 
{|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
 
{|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
−
|width='340px'|'''Characteristics'''
+
|width='680px' align="left" colspan="4"|'''Characteristics'''
−
{|align="left"
+
|-
−
|width='120px'|[[Input Swing|''Input Swing:'']]
+
|width='80px'|[[Input Swing|''Input Swing:'']]
−
|width='210px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+
|width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 +
|width='130px' align='left'|[[Resonse time|''L/H Response time:'']]
 +
|width='210px' align='left'|25 mins
 
|-
 
|-
 
|[[Output Swing|''Output Swing:'']]
 
|[[Output Swing|''Output Swing:'']]
 
|13.6 - 490.2 GFP molecules/s.cfu
 
|13.6 - 490.2 GFP molecules/s.cfu
 +
|align='left'|[[Genetic Stability|''Genetic Stability:'']]
 +
|92/74 generations (Low/High Input)
 
|-
 
|-
 
|[[Switch Point|''Switch Point:'']]
 
|[[Switch Point|''Switch Point:'']]
−
|6 nM 3OC<sub>6</sub>HSL, exogenous
+
|6 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
−
|-
+
|align='left'|[[Performance Stability|''Performance Stability:'']]
−
|[[Latency|''L/H Response time:'']]
+
|92/74 generations (Low/High Input)
−
|25 mins
+
−
|}
+
−
|width='340px' align="right" valign="top"|'''Key Subparts'''<br>
+
−
{|align="right"
+
−
|width='85px'|''<partinfo>R0040</partinfo>:''
+
−
|TetR-regulated operator
+
−
|-
+
−
|''<partinfo>C0062</partinfo>:''
+
−
|luxR ORF
+
−
|-
+
−
|''<partinfo>R0062</partinfo>:''
+
−
|LuxR-regulated operator<br>
+
−
|}
+
 
|}
 
|}
 
{|align="center" cellspacing="0" cellpadding="0"
 
{|align="center" cellspacing="0" cellpadding="0"
−
|width='375px' align="left"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]<br>
+
|width='340px' align="left"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]<br>
−
{{Click || image=BC-TransferCurve-3.png | link=Part:BBa_F2620:Transfer Function |width=300px | height=200px }}
+
{{Click || image=BC-TransferCurve-3.png | link=Part:BBa_F2620:Transfer Function |width=320px | height=200px }}
−
|width='375px' align="right"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br>
+
|width='340px' align="left"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br>
−
{{Click || image=BC-Specificity-1.png | link=Part:BBa_F2620:Specificity |width=300px | height=200px }}
+
{{Click || image=BC-Specificity-1.png | link=Part:BBa_F2620:Specificity |width=320px | height=200px }}
 
|-
 
|-
 
|align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br>
 
|align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br>
−
{{Click || image=BC-Latency-2.png | link=Part:BBa_F2620:Response time |width=300px | height=200px }}
+
{{Click || image=BC-Latency-2.png | link=Part:BBa_F2620:Response time |width=320px | height=200px }}
−
|align="right"|[[Part:BBa_F2620:Stability|'''Stability''']]<br>
+
|align="left"|[[Part:BBa_F2620:Stability|'''Stability''']]<br><center>
−
{{Click || image=BC-Stability-1.png | link=Part:BBa_F2620:Stability |width=300px | height=250px }}
+
{{Click || image=BC-Stability-1.png | link=Part:BBa_F2620:Stability |width=250px | height=200px }}</center>
 
|}
 
|}
 +
<br>
 
<div style="padding: 00px; width: 680px">
 
<div style="padding: 00px; width: 680px">
 
{|align="center" cellspacing="0" cellpadding="0"  
 
{|align="center" cellspacing="0" cellpadding="0"  
−
|width='340px'  valign="top"|'''Demand'''
+
|width='680px'  colspan="4" valign="top"|'''Demand'''
−
{|align="left"
+
−
|width='125px' valign="top"|[[Transcription Demand|''Transcription Demand:'']]
+
−
|width='215px'valign="top"|## NTP per second<br>## polymerases sequestered
+
 
|-
 
|-
−
|valign="top"| [[Translation Demand|''Translation Demand:'']]
+
|width='130px' valign="top"|[[Transcription Demand|''Transcription Demand:'']]
−
|valign="top"| # charged tRNA per second<br># ribosomes sequestered
+
|width='210px' valign="top"|## NTP per second<br>## polymerases sequestered
−
|}
+
|width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]
−
|width='340px' align="right" valign="top"|'''Stability'''<br>
+
|width='215px' valign="top"| # charged tRNA per second<br># ribosomes sequestered
−
{|align="right"
+
−
|width='120px' valign="top"|[[Genetic Stability|''Genetic Stability:'']]
+
−
|valign="top"| 92/74 generations (Low/High Input)
+
−
|-
+
−
|valign="top"|[[Performance Stability|''Performance Stability:'']]
+
−
|valign="top"| 92/74 generations (Low/High Input)
+
−
|}
+
 
|}
 
|}
 
</div>
 
</div>
Line 69: Line 49:
 
'''Compatibility'''<br>
 
'''Compatibility'''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br>
−
[[Plasmids|''Plasmid:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br>
+
[[Plasmids|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br>
−
[[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'',''<partinfo>BBa_E0434</partinfo>'',''<partinfo>BBa_E0240</partinfo>''<br>
+
[[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'', ''<partinfo>BBa_E0434</partinfo>'', ''<partinfo>BBa_E0240</partinfo>''<br>
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
 
Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br>
 
Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br>
 
</div>
 
</div>
 
</div>
 
</div>
−
[[Image:RoSBP Banner.jpg|680px]]
 

Revision as of 00:33, 4 October 2006

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: 0.1 - 1000 nM 3OC6HSL, exogenous L/H Response time: 25 mins
Output Swing: 13.6 - 490.2 GFP molecules/s.cfu Genetic Stability: 92/74 generations (Low/High Input)
Switch Point: 6 nM 3OC6HSL, exogenous Performance Stability: 92/74 generations (Low/High Input)
Transfer Function
 Specificity
Response time
 Stability


Demand
Transcription Demand: ## NTP per second
## polymerases sequestered 		Translation Demand: # charged tRNA per second
# ribosomes sequestered

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing BBa_C0040.