Difference between revisions of "Part:BBa K1483000:Design"
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===References=== | ===References=== | ||
− | The sequence is based on data from | + | The sequence is based on data from |
Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464. | Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464. |
Revision as of 22:05, 6 October 2014
α-N-Acetylgalactosamindase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 405
Design Notes
Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
Source
The protein encoded by this sequence naturally occurs in Elizabethkingia meningoseptica(syn.: Chryseobacterium meningosepticum or Flavobacterium meningosepticum). This sequence was optimized for codon usage in E. coli K12 and synthesized.
References
The sequence is based on data from Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.