Difference between revisions of "Part:BBa K1448000"

(Picture of our designned part)
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== '''Characterization''' ==
 
== '''Characterization''' ==
=== Picture of our designned part ===
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=== Gel picture of our designned part ===
 
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Revision as of 21:54, 6 October 2014

gamma-terpinene synthase

gamma-terpinene synthase produces gamma-terpinene from GPP,geranyl diphosphate.

This part contains gamma-terpinene synthase region of Citrus unshiu and was isolated by Mr.Shimada from Citrus unshiu.http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003


Background and prnciples

Characterization

Gel picture of our designned part


Westernbloting analysis

Fig1,Western blot analysis of GST and d-limonene synthase(Lane2), ɤ-terpinene synthase(Lane3) and β-pinene synthases(Lane4) fusion proteins expressed by yeast. Lanes: 1, p427-TEF; 2, p427-TEF-LS; 3, p427-TEF-gTS; 4, p427-TEF-PS.

Reference


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 204
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]