Difference between revisions of "Part:BBa K1489000:Design"

Revision as of 20:44, 6 October 2014

pBAD-RBS-Bovine Galectin-1-B0012 Terminator

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal XhoI site found at 499
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

EcoRI site in middle of Bovine Galectin gene was removed, codons were optimized for expression in E. coli.

Source

Sequence generated from Bos taurus genome, reoptimized for E. coli expression.

References

Bourne Y, Bolgiano B, Liao DI, et al. Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol. 1994;1(12):863-70.

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	===References===		===References===
		+	Bourne Y, Bolgiano B, Liao DI, et al. Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol. 1994;1(12):863-70.