Difference between revisions of "Part:BBa K1403001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1403001 short</partinfo>
 
<partinfo>BBa_K1403001 short</partinfo>
  
 
<partinfo>BBa_K1403001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1403001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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nk
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The designed was made according to interlab study requirements. The promoter and the vector have been taken from [[Part:BBa_BBa_K823012|BBa_BBa_K823012]], cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from [[Part:BBa_E0240|BBa_E0240]] also with XbeI and SpeI and introduced to the cut promoter + backbone.
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We used heatshock competent NEB cels.
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For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]
  
 
===Source===
 
===Source===
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<br/>
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Interlab study
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kit 2014
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<br/>
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[[Part:BBa_K823012|BBa_K823012]]: Plate 1, Well 20K
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<br/>
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[[Part:BBa_E0240|BBa_E0240]]: Plate 1, Well 22I
  
 
===References===
 
===References===
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http://2014.igem.org/Tracks/Measurement/Interlab_study

Latest revision as of 19:08, 6 October 2014

GFP generator in pSB1C3 under a weak promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

The designed was made according to interlab study requirements. The promoter and the vector have been taken from BBa_BBa_K823012, cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from BBa_E0240 also with XbeI and SpeI and introduced to the cut promoter + backbone. We used heatshock competent NEB cels. For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]

Source


kit 2014
BBa_K823012: Plate 1, Well 20K
BBa_E0240: Plate 1, Well 22I

References

http://2014.igem.org/Tracks/Measurement/Interlab_study