Difference between revisions of "Part:BBa J23100"
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| + | '''Constitutive promoter family''' | ||
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use. | Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use. | ||
| − | These promoter parts can be used to tune the expression level of constitutively expressed parts. | + | These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for the construction of synthetic promoters. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:03, 3 October 2006
constitutive promoter family member
Variant RFP (au)
J23112 1
J23103 17 J23107 908
J23113 21 J23106 1185
J23109 106 J23108 1303
J23117 162 J23118 1429
J23114 256 J23111 1487
J23115 387 J23101 1791
J23116 396 J23104 1831
J23105 623 J23102 2179
J23110 844 J23100 2547
Constitutive promoter family Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use.
These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for the construction of synthetic promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]