Difference between revisions of "Part:BBa F2620"
Line 3: Line 3:
 
|Last Update=15th Sept. 2006
 
|Last Update=15th Sept. 2006
 
}}
 
}}
−
{{Template:F2620 Characterization Navbar}}
+
{{Template:F2620 Characterization Navbar}}<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
−
<div style="padding: 00px; width: 700px; border: 0px solid #000000;">
+
 
'''Description'''<br>
 
'''Description'''<br>
−
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.
+
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
  
−
'''Usage'''<br>
 
−
Full [[PoPS]] output at high [[3OC6HSL]] levels and high plasmid copy [e.g., [[Part:pSB1A2|pSB1A2]]] results in a reduced cell growth rate (see Load section).  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.   
 
 
</div>
 
</div>
 
{|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
 
{|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
−
|width='360px'|'''Characteristics'''
+
|width='340px'|'''Characteristics'''
 
{|align="left"
 
{|align="left"
 
|width='85px'|[[Input Swing|''Input Swing:'']]
 
|width='85px'|[[Input Swing|''Input Swing:'']]
−
|width='265px'|## nM 3OC<sub>6</sub>HSL, exogenous
+
|width='245px'|0.1 - 1000 nM 3OC<sub>6</sub>HSL, exogenous
 
|-
 
|-
 
|[[Output Swing|''Output Swing:'']]
 
|[[Output Swing|''Output Swing:'']]
−
|#-# PoPS
+
|13.6 - 490.2 GFP molecules/s.cfu
 
|-
 
|-
 
|[[Switch Point|''Switch Point:'']]
 
|[[Switch Point|''Switch Point:'']]
−
|2 nM 3OC<sub>6</sub>HSL, exogenous
+
|6 nM 3OC<sub>6</sub>HSL, exogenous
 
|-
 
|-
 
|[[Latency|''L/H Latency:'']]
 
|[[Latency|''L/H Latency:'']]
−
|# minutes
+
|25 mins
 
|}
 
|}
−
|width='360px' align="right" valign="top"|'''Key Subparts'''<br>
+
|width='340px' align="right" valign="top"|'''Key Subparts'''<br>
 
{|align="right"
 
{|align="right"
 
|width='85px'|''<partinfo>R0040</partinfo>:''
 
|width='85px'|''<partinfo>R0040</partinfo>:''
Line 38: Line 35:
 
|}
 
|}
 
|}
 
|}
−
{|align="center" cellspacing="0" cellpadding="0"
+
<div style="padding: 00px; width: 680px">
−
|width='375px' align="left"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]<br>
+
−
{{Click || image=BC-TransferCurve-3.png | link=Part:BBa_F2620:Transfer Function |width=300px | height=200px }}
+
−
|width='375px' align="right"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br>
+
−
{{Click || image=BC-Specificity-1.png | link=Part:BBa_F2620:Specificity |width=300px | height=200px }}
+
−
|-
+
−
|align="left"|[[Part:BBa_F2620:Latency|'''Latency''']]<br>
+
−
{{Click || image=BC-Latency-2.png | link=Part:BBa_F2620:Latency |width=300px | height=200px }}
+
−
|align="right"|[[Part:BBa_F2620:Stability|'''Stability''']]<br>
+
−
{{Click || image=BC-Stability-1.png | link=Part:BBa_F2620:Stability |width=300px | height=250px }}
+
−
|}
+
−
<div style="padding: 00px; width: 720px">
+
 
{|align="center" cellspacing="0" cellpadding="0"  
 
{|align="center" cellspacing="0" cellpadding="0"  
−
|width='360px'  valign="top"|'''Demand'''
+
|width='340px'  valign="top"|'''Demand'''
 
{|align="left"
 
{|align="left"
 
|width='125px' valign="top"|[[Transcription Demand|''Transcription Demand:'']]
 
|width='125px' valign="top"|[[Transcription Demand|''Transcription Demand:'']]
−
|width='235px'valign="top"|## NTP per second<br>## polymerases sequestered
+
|width='215px'valign="top"|## NTP per second<br>## polymerases sequestered
 
|-
 
|-
 
|valign="top"| [[Translation Demand|''Translation Demand:'']]
 
|valign="top"| [[Translation Demand|''Translation Demand:'']]
 
|valign="top"| # charged tRNA per second<br># ribosomes sequestered
 
|valign="top"| # charged tRNA per second<br># ribosomes sequestered
 
|}
 
|}
−
|width='360px' align="right" valign="top"|'''Stability'''<br>
+
|width='340px' align="right" valign="top"|'''Stability'''<br>
 
{|align="right"
 
{|align="right"
−
|width='140px' valign="top"|[[Genetic Stability|''Genetic Stability:'']]
+
|width='120px' valign="top"|[[Genetic Stability|''Genetic Stability:'']]
−
|valign="top"| ##/## replication events (Lo Input/High Input)
+
|valign="top"| 92/74 generations (Low/High Input)
 
|-
 
|-
 
|valign="top"|[[Performance Stability|''Performance Stability:'']]
 
|valign="top"|[[Performance Stability|''Performance Stability:'']]
−
|valign="top"|##/## replication events (Lo Input/High Input)
+
|valign="top"| 92/74 generations (Low/High Input)
 
|}
 
|}
 
|}
 
|}
Line 72: Line 58:
 
<div style="padding: 00px; width: 720px">
 
<div style="padding: 00px; width: 720px">
 
'''Compatibility'''<br>
 
'''Compatibility'''<br>
−
Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br>
+
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br>
−
Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB3k3</partinfo>''<br>
+
[[Plasmids|''Plasmid:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br>
−
Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'',''<partinfo>BBa_E0434</partinfo>'',''<partinfo>BBa_E0240</partinfo>''<br>
+
[[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'',''<partinfo>BBa_E0434</partinfo>'',''<partinfo>BBa_E0240</partinfo>''<br>
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
 
Crosstalk with systems containing ''TetR'' (''<partinfo>BBa_C0040</partinfo>'')<br>
 
Crosstalk with systems containing ''TetR'' (''<partinfo>BBa_C0040</partinfo>'')<br>
Line 81: Line 67:
 
</div>
 
</div>
 
</div>
 
</div>
−
[[Image:RoSBP Banner.jpg|800px]]
+
[[Image:RoSBP Banner.jpg|762px]]

Revision as of 22:00, 3 October 2006

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Part Main Page        Transfer Function        Specificity        Response time        Stability        Add Data       


Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: 0.1 - 1000 nM 3OC6HSL, exogenous
Output Swing: 13.6 - 490.2 GFP molecules/s.cfu
Switch Point: 6 nM 3OC6HSL, exogenous
L/H Latency: 25 mins
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator
Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: 92/74 generations (Low/High Input)
Performance Stability: 92/74 generations (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmid: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

RoSBP Banner.jpg