Difference between revisions of "Part:BBa K1442101"
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| − | To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga). | + | To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga). Therefore, the region beyond said codon remains untranslated, so the phenotype of this mutant is determined entirely by the region previous to the stop codon. |
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Revision as of 16:06, 6 October 2014
RdRP Mutant
To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga). Therefore, the region beyond said codon remains untranslated, so the phenotype of this mutant is determined entirely by the region previous to the stop codon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 492
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]