Difference between revisions of "Part:BBa K1351015"
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Quorum sensing two component system with native RBS and Terminator (histidine kinase comD, response regulator comE) of ''Streptococcus pneumoniae'' (R6) able to sense the competence stimulating peptide CSP.  
 
Quorum sensing two component system with native RBS and Terminator (histidine kinase comD, response regulator comE) of ''Streptococcus pneumoniae'' (R6) able to sense the competence stimulating peptide CSP.  
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<br> Präfix: GATCGAATTCGCGGCCGCTTCTAGAGGAAATAAGGGGAAAGAGTAATGG
+
 
 +
<br><br>Used oligonucleotides
 +
<br> Prefix: GATCGAATTCGCGGCCGCTTCTAGAGGAAATAAGGGGAAAGAGTAATGG
 
<br> Sufix: GATCTACTAGTAGCGGCCGCTGCAGATAGTCCGTACGGGATTCG
 
<br> Sufix: GATCTACTAGTAGCGGCCGCTGCAGATAGTCCGTACGGGATTCG
  

Revision as of 15:28, 6 October 2014

comDE Quorum Sensing two component system - native

Quorum sensing two component system with native RBS and Terminator (histidine kinase comD, response regulator comE) of Streptococcus pneumoniae (R6) able to sense the competence stimulating peptide CSP.



Used oligonucleotides
Prefix: GATCGAATTCGCGGCCGCTTCTAGAGGAAATAAGGGGAAAGAGTAATGG
Sufix: GATCTACTAGTAGCGGCCGCTGCAGATAGTCCGTACGGGATTCG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]