Difference between revisions of "Part:BBa K1351016"
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Quorum sensing two component system (histidine kinase comD, response regulator comE)of ''Streptococcus pneumoniae'' (R6) able to sense the competence stimulating peptide CSP. Part contains optimized RBS and Terminator for Bacillus subtilis.
 
Quorum sensing two component system (histidine kinase comD, response regulator comE)of ''Streptococcus pneumoniae'' (R6) able to sense the competence stimulating peptide CSP. Part contains optimized RBS and Terminator for Bacillus subtilis.
  
−
fwd primer:gatcgaattccgcggccgcttctagataaggaggagccggcATGGATTTATTTGGATTTGGGACGG
+
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:  
−
rev primer:gatcctgcagcggccgctactagtattaaccggtCTTTTGAGATTTTTTCTCTAAAATATC
+
 
 +
{|
 +
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
 +
|<span style="color:blue">GAATTCC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>T<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>
 +
|-
 +
|suffix with AgeI, SpeI, NotI and PstI:
 +
|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
 +
|}
 +
Sites of restriction enzymes generating compatible overhangs have the same color:
 +
 
 +
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Shine-Dalgarno sequence and stop codons are underlined.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:13, 6 October 2014

comDE Quorum Sensing two component system (Freiburg Standard)

Quorum sensing two component system (histidine kinase comD, response regulator comE)of Streptococcus pneumoniae (R6) able to sense the competence stimulating peptide CSP. Part contains optimized RBS and Terminator for Bacillus subtilis.

This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI, SD and NgoMIV: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]