Difference between revisions of "Part:BBa K1351025"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1351025 short</partinfo> | <partinfo>BBa_K1351025 short</partinfo> | ||
| − | CSP-depending promoter of Streptococcus pneumoniae. | + | CSP-depending promoter of ''Streptococcus pneumoniae''. |
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: | This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: | ||
Revision as of 15:03, 6 October 2014
PcomAB, a CSP inducible promoter derived from Streptococcus pneumoniae
CSP-depending promoter of Streptococcus pneumoniae.
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
| prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | GAATTCCGCGGCCGCTTCTAGATAAGGAGGAGCCGGC |
| suffix with AgeI, SpeI, NotI and PstI: | ACCGGTTAATACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]