Difference between revisions of "Part:BBa K1350006:Experience"

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'''==  Characterization experiment on BBa_K1350006--Mdfa =='''
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''''''==  Characterization experiment on BBa_K1350006--Mdfa =='''
  
 
   Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
 
   Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
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   3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 
   3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 
   3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 
   3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
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'''

Revision as of 11:59, 6 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1350006

User Reviews

UNIQ009a148ef5de9b13-partinfo-00000000-QINU UNIQ009a148ef5de9b13-partinfo-00000001-QINU


'== Characterization experiment on BBa_K1350006--Mdfa == 

 Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
 We performed some experiments to prove that the Mdfa really founctions.
 We performed several experiments with different LB medium. We used: 
 
 3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5