Difference between revisions of "Part:BBa K1350006:Experience"

 
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==  Characterization experiment on BBa_K1350006--Mdfa ==
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  Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
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  We performed some experiments to prove that the Mdfa really founctions.
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  We performed several experiments with different LB medium. We used:
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  3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
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  3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5

Revision as of 11:58, 6 October 2014


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Characterization experiment on BBa_K1350006--Mdfa

 Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
 We performed some experiments to prove that the Mdfa really founctions.
 We performed several experiments with different LB medium. We used: 
 
 3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5