Difference between revisions of "Part:BBa K1442023:Design"

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===Design Notes===
 
===Design Notes===
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An AscI and BsrGI restriction site were added at the beginning and end of the sequence respectively in order to clone this part into our testing modules.  
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<p>An AscI and BsrGI restriction site were added at the beginning and end of the sequence respectively in order to clone this part into our testing modules. </p>
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'''AscI''' is compatible with MluI
+
<p>'''AscI''' is compatible with MluI </p>
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'''BsrGI'''is compatible with BsiWI and TatI
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<p>'''BsrGI'''is compatible with BsiWI and TatI </p>
  
 
===Source===
 
===Source===

Revision as of 10:19, 6 October 2014

EMCV IRES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An AscI and BsrGI restriction site were added at the beginning and end of the sequence respectively in order to clone this part into our testing modules.

AscI is compatible with MluI

BsrGIis compatible with BsiWI and TatI

Source

The IRES came from the Encephalomyocarditis virus (EMCV) virus.

References

[1] Bochkov and Palmenberg, 2006, Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location, BioTechniques, 41:283-292 (September 2006) http://www.biotechniques.com/multimedia/archive/00002/BTN_A_000112243_O_2391a.pdf