Difference between revisions of "Part:BBa K1362992:Design"
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===Source=== | ===Source=== | ||
− | Npu DnaE C-Intein cloned from pVS41 obtained from Prof. Henning D. Mootz, University of Muenster. BBa_J04450 was used as mRFP selection marker. | + | The RBS comes from BBa_K1362090. Npu DnaE C-Intein was cloned from pVS41 obtained from Prof. Henning D. Mootz, University of Muenster. BBa_J04450 was used as mRFP selection marker. |
===References=== | ===References=== |
Latest revision as of 23:25, 5 October 2014
-- No description --
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Part was designed to be used with to RFC[???]. Parts were amplified from source plasmids with matching overhangs and assembled via CPEC.
Source
The RBS comes from BBa_K1362090. Npu DnaE C-Intein was cloned from pVS41 obtained from Prof. Henning D. Mootz, University of Muenster. BBa_J04450 was used as mRFP selection marker.