Difference between revisions of "Part:BBa K1465104:Design"

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===Design Notes===
 
===Design Notes===
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*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_BCD pSB1C3_pre_Fum_BCD] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_BCD pSB1C3_suf_Fum_BCD]:<br>
  
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'''pSB1C3_pre_Fum_BCD (42 bp)'''<br>
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5'-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3'<br>
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'''pSB1C3_suf_Fum_BCD (43 bp)'''<br>
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5'-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the subunites B,C and D of the Fumerate reductase from ''Actinobacillus succinogenes''.<br>
  
 
===Source===
 
===Source===

Latest revision as of 22:32, 5 October 2014


Fumarate reductase FumBCD from Actinobacillus succinogenes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
    Illegal AgeI site found at 1230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1146


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_BCD pSB1C3_pre_Fum_BCD] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_BCD pSB1C3_suf_Fum_BCD]:
pSB1C3_pre_Fum_BCD (42 bp)

5'-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3'

pSB1C3_suf_Fum_BCD (43 bp)

5'-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the subunites B,C and D of the Fumerate reductase from Actinobacillus succinogenes.

Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the codon optimized sequence for Escherchia coli by using the [http://www.jcat.de/ Codon Adaptation Tool] and the [http://eu.idtdna.com/CodonOpt Codon Optimization from IDT]:


BILD

References