Difference between revisions of "Part:BBa K1362090"
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RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.
 
RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.
  
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The RBS sequence is identical to that of <partinfo>BBa_J18912 </partinfo> and expression can be expected to be comparable.
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<i>The</i> ''RBS'' sequence is identical to that of <partinfo>BBa_J18912 </partinfo> and expression can be expected to be comparable.
  
 
<partinfo>BBa_K1362090 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1362090 SequenceAndFeatures</partinfo>

Revision as of 21:26, 5 October 2014

RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.

The RBS sequence is identical to that of BBa_J18912 and expression can be expected to be comparable.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. Olins, P. O. & Rangwala, S. H. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264, 16973–6 (1989).