Difference between revisions of "Part:BBa K1412008"

Revision as of 16:08, 5 October 2014

Drive the E.coli engineered strain(CL-1) towards death region

What it is

Composite part enables the engineered bacteria swim to killing osmotic pressure which will kill bacteria itself. pay attention that this is a truncated version of BBa_K1412010.

How it works

E.coli make use of the EnvZ/OmpR system to mediate signal transduction in response to environmental osmolarity changes. EnvZ, a histidine kinase, undergoes trans-autophosphorylation, then the high energy phosphoryl group is subsequently transferred to OmpR, a response regulator. In the system, OmpR-controlled promoter (PompC) is involved in. The expression strength of PompC is depending upon the medium osmolarity. When medium osmolarity is increasing, the EnvZ will phosphorylate more OmpR into phosphorylated OmpR (OmpR-P), and more OmpR-P will result in stronger expression strength of PompC. In our circuitry design, CheZ is upstream regulated by PompC. As the osmotic pressure is increasing, the motile ability of the engineered E.coli keeps growing, resulting in it's suicide.

What is the difference

Comparing with the biobrick BBa_K1412010, you could find that within BBa_K1412008, the promoter BBa_R0082 is replaced by BBa_R0083. As a result, the length of the device is shortened while the efficiency decreases. (More details about the biobricks BBa_R0082 and BBa_R0083.)

Sequence and Features

Reference