Difference between revisions of "Part:BBa K1484001"

Revision as of 16:02, 5 October 2014

tsPurple chromoprotein in GoldenGate standard

Origin:
tsPurple is a chromoprotein which takes its name from TinselPurple. It exhibits a strong purple colour when expressed. The protein has its maximum absorption at ***nm giving it a purple hue visible to the naked eye. (visible wavelength: 390 to 700nm)

Expression:
The chromoprotein can be readily observed when expressed in E.coli in both LB or agar plate cultures within 24h of inoculation. This protein has also been successfully expressed in Marchantia polymorpha, a plant chassis.

GoldenGate Standards:
This version of tsPurple is compatible with the GoldenGate standards as described in the 'design section' which allows more efficient assembly of parts. The protein structure or expression pattern is unchanged by this alteration which only affects the sequence before and after the CDS.

Origin:
The protein was sourced from the pre-existing biobrick: BBa_K1033906

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 704

@@ Line 16: / Line 16: @@
 This version of tsPurple is compatible with the GoldenGate standards as described in the 'design section' which allows more efficient assembly of parts. The protein structure or expression pattern is unchanged by this alteration which only affects the sequence before and after the CDS.
+'''Origin:'''
+<br>
+The protein was sourced from the pre-existing biobrick:
+BBa_K1033906
 <!-- Add more about the biology of this part here