Difference between revisions of "Part:BBa K1484001"
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tsPurple is a chromoprotein which takes its name from TinselPurple. It exhibits a strong purple colour when expressed. | tsPurple is a chromoprotein which takes its name from TinselPurple. It exhibits a strong purple colour when expressed. | ||
The protein has its maximum absorption at ***nm giving it a purple hue visible to the naked eye. (visible wavelength: 390 to 700nm) | The protein has its maximum absorption at ***nm giving it a purple hue visible to the naked eye. (visible wavelength: 390 to 700nm) | ||
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'''Expression:''' | '''Expression:''' | ||
Revision as of 15:55, 5 October 2014
tsPurple chromoprotein in GoldenGate standard
Origin:
tsPurple is a chromoprotein which takes its name from TinselPurple. It exhibits a strong purple colour when expressed.
The protein has its maximum absorption at ***nm giving it a purple hue visible to the naked eye. (visible wavelength: 390 to 700nm)
Expression:
The chromoprotein can be readily observed when expressed in E.coli in both LB or agar plate cultures within 24h of inoculation.
This protein has also been successfully expressed in Marchantia polymorpha, a plant chassis.
GoldenGate Standards:
This version of tsPurple is compatible with the GoldenGate standards as described in the 'design section' which allows more efficient assembly of parts. The protein structure or expression pattern is unchanged by this alteration which only affects the sequence before and after the CDS.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 704