Difference between revisions of "Part:BBa K1351008:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351008 short</partinfo>
 
<partinfo>BBa_K1351008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The native signal peptide of YhcR was used instead of known Sec-dependent signal peptides as Liew ''et. al.'' did 2012.
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The native signal peptide of YhcR was used instead of known Sec-dependent signal peptides as in Liew ''et. al.'', 2012.
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===Source===
 
===Source===
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SP_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGCTGTCTGTCGAAATGATAAGC  
 
SP_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGCTGTCTGTCGAAATGATAAGC  
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SP_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT AGCTTCGAACGTGTACATTACATTTAAG
 
SP_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT AGCTTCGAACGTGTACATTACATTTAAG
  
 
===References===
 
===References===
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Liew, P. X., C. L. Wang and S. L. Wong. "Functional Characterization and Localization of a Bacillus Subtilis Sortase and Its Substrate and Use of This Sortase System to Covalently Anchor a Heterologous Protein to the B. Subtilis Cell Wall for Surface Display." J Bacteriol 194, no. 1 (2012): 161-75. [http://www.ncbi.nlm.nih.gov/pubmed/22020651 PubMed]
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Tjalsma, H., H. Antelmann, J. D. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J. Y. Dubois, H. Westers, G. Zanen, W. J. Quax, O. P. Kuipers, S. Bron, M. Hecker and J. M. van Dijl. "Proteomics of Protein Secretion by Bacillus Subtilis: Separating the "Secrets" of the Secretome." Microbiol Mol Biol Rev 68, no. 2 (2004): 207-33. [http://www.ncbi.nlm.nih.gov/pubmed/15187182 PubMed]

Revision as of 13:00, 5 October 2014

Signal peptide of B. subtilis endonuclease YhcR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native signal peptide of YhcR was used instead of known Sec-dependent signal peptides as in Liew et. al., 2012.

Source

This part was generated by amplification from B. subtilis W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

SP_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGCTGTCTGTCGAAATGATAAGC SP_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT AGCTTCGAACGTGTACATTACATTTAAG

References

Liew, P. X., C. L. Wang and S. L. Wong. "Functional Characterization and Localization of a Bacillus Subtilis Sortase and Its Substrate and Use of This Sortase System to Covalently Anchor a Heterologous Protein to the B. Subtilis Cell Wall for Surface Display." J Bacteriol 194, no. 1 (2012): 161-75. [http://www.ncbi.nlm.nih.gov/pubmed/22020651 PubMed]

Tjalsma, H., H. Antelmann, J. D. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J. Y. Dubois, H. Westers, G. Zanen, W. J. Quax, O. P. Kuipers, S. Bron, M. Hecker and J. M. van Dijl. "Proteomics of Protein Secretion by Bacillus Subtilis: Separating the "Secrets" of the Secretome." Microbiol Mol Biol Rev 68, no. 2 (2004): 207-33. [http://www.ncbi.nlm.nih.gov/pubmed/15187182 PubMed]