Difference between revisions of "Part:BBa K1362090"

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The RBS sequence is identical to that of [[Part:BBa_J18912|BBa_J18912]] and expression can be expected to be comparable.
 
The RBS sequence is identical to that of [[Part:BBa_J18912|BBa_J18912]] and expression can be expected to be comparable.
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<partinfo>BBa_K1362990 SequenceAndFeatures</partinfo>
  
 
==References==
 
==References==
 
1. Olins, P. O. & Rangwala, S. H. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264, 16973–6 (1989).
 
1. Olins, P. O. & Rangwala, S. H. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264, 16973–6 (1989).

Revision as of 03:29, 5 October 2014

RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.

The RBS sequence is identical to that of BBa_J18912 and expression can be expected to be comparable.

No part name specified with partinfo tag.

References

1. Olins, P. O. & Rangwala, S. H. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264, 16973–6 (1989).