Revision as of 14:51, 4 October 2014

Basic part of biobrick BBa_K1412001

What it is

Basic part of biobrick K1412001, lacking of promoter BBa_K206000(pTET).

What it does

Through ligating this part with different promoters such as pBAD, pTET, pCONS and transform the plasmid into E.coli(CL-1), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the E.coli(CL-1) under our command to form different pattern which is mathematically meaning.

How to use it

After ligated with promoter,we could transform this part into CL-1 to obtain the engineering bacteria. Incubate them on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and got the experimental data.

Experimental Data

With K20600(pBAD) as an inhibit promoter

protocol

1.Transformation

2.Extract plasmids

3.Digestion

4.DNA gel electrophoresis

5.Gel Extraction

6.Ligation

7.Transformation

8.Extract plasmids

9.Digestion

10.DNA gel electrophoresis

11.Transform the plasmid into CL-1

Sequence and Features