Difference between revisions of "Part:BBa K1412002"
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== What it is == | == What it is == | ||
| − | + | Basic part of biobrick K1412001, lacking of promoter BBa_K206000(ptet). | |
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== What it does == | == What it does == | ||
Revision as of 14:36, 4 October 2014
Contents
Basic part of biobrick BBa_K1412001
What it is
Basic part of biobrick K1412001, lacking of promoter BBa_K206000(ptet).
What it does
As a control group for K1412001, we use this part to eliminate background expression of AraC, and it’s also capable to form ellipse on the plate under the induction of IPTG.
How to use it
Transforming this part into CL-1, we got the engineering bacteria. Incubate them on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and got the experimental data. It is also used to form ellipse.
Experimental Data
With K20600(pBAD) as an inhibit promoter
protocol
1.Transformation
2.Extract plasmids
3.Digestion
4.DNA gel electrophoresis
5.Gel Extraction
6.Ligation
7.Transformation
8.Extract plasmids
9.Digestion
10.DNA gel electrophoresis
11.Transform the plasmid into CL-1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]