Difference between revisions of "Part:BBa K1402001"

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ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans.this ABC transporters MsbA can transport fatty acids out of the cell.The RBS is in front of the MsbA coding sequence and T7 terminator is after MsbA. You can design your own promoter for this part ,but use this part in E.coli BL21(DE3) only or you’d better delete the T7 terminator.
 
ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans.this ABC transporters MsbA can transport fatty acids out of the cell.The RBS is in front of the MsbA coding sequence and T7 terminator is after MsbA. You can design your own promoter for this part ,but use this part in E.coli BL21(DE3) only or you’d better delete the T7 terminator.

Latest revision as of 14:01, 4 October 2014

RBS-MsbA-Terminator

ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans.this ABC transporters MsbA can transport fatty acids out of the cell.The RBS is in front of the MsbA coding sequence and T7 terminator is after MsbA. You can design your own promoter for this part ,but use this part in E.coli BL21(DE3) only or you’d better delete the T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 27
    Illegal XhoI site found at 1487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1417