Difference between revisions of "Part:BBa K1402016"

Latest revision as of 13:43, 4 October 2014

Ara-RBS-BTE-Treminator

Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.The RBS and ara promoter are before the BTE coding sequence and T7 terminator is after BTE. Ara promoter is a direct copy of Pbad promoter in E.Coli DH5α,the natural promoter for the Ara operon.We can activate the expression of BTE by adding arabinose in the culture in the absence of glucose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 797
Illegal BglII site found at 937
Illegal BamHI site found at 236
Illegal BamHI site found at 386
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 71
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1346
Illegal SapI site found at 53

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1402016 short</partinfo>
-This part contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds AraC protein.In the absence of AraC protein and CAP protein, this part promotes transcription.In the presence of AraC protein and CAP protein, this part inhibits transcription.This is a direct copy of Pbad promoter in E.coli DH5&#945; ,the natural promoter for the Ara operon.
+Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.The RBS and ara promoter are before the BTE coding sequence and T7 terminator is after BTE. Ara promoter is a direct copy of Pbad promoter in E.Coli DH5α,the natural promoter for the Ara operon.We can activate the expression of BTE by adding arabinose in the culture in the absence of glucose.