Difference between revisions of "Part:BBa K1465103:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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gblocks
 
  
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*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_A pSB1C3_pre_Fum_A] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_A pSB1C3_suf_Fum_A]:<br>
  
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'''pSB1C3_pre_Fum_A (43 bp)'''<br>
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5'-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'<br>
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'''pSB1C3_suf_FumA (43 bp)'''<br>
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5'-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Fumerate reductase A (''FumA'') from ''Actinobacillus succinogenes''.<br>
  
 
===Source===
 
===Source===

Revision as of 22:35, 3 October 2014


Fumarate reductase subunit A (FumA) from Actinobacillus succinogenes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 734
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1120


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_A pSB1C3_pre_Fum_A] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_A pSB1C3_suf_Fum_A]:
pSB1C3_pre_Fum_A (43 bp)

5'-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_FumA (43 bp)

5'-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Fumerate reductase A (FumA) from Actinobacillus succinogenes.

Source

Gibson

References