Difference between revisions of "Part:BBa J61023:Design"

 
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Product is Bca1014  *internal PstI and NotI sites
 
Product is Bca1014  *internal PstI and NotI sites
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+
----
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+
ca1014F Forward EcoRI/XbaI for Tnp biobrick<br>
 +
gcaatGAATTCTCTAGAATGATTCTCCGCCAG<br>
 +
ca1014R Reverse SpeI for Tnp biobrick<br>
 +
cctatactagtCGACGGATCAGATCTTGATC<br>
  
 
===References===
 
===References===

Latest revision as of 23:59, 21 September 2006


Tn5 Transposase [Tnp1-PN] (with PstI and NotI)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1220
    Illegal PstI site found at 885
    Illegal NotI site found at 798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
    Illegal XhoI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 885
    Illegal NgoMIV site found at 73
    Illegal AgeI site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1352
    Illegal BsaI.rc site found at 1173
    Illegal SapI site found at 309
    Illegal SapI.rc site found at 621


Design Notes

There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.


Source

PCR ca1014F/R on pRL27 (1763 bp, EcoRI/SpeI)

Sub into pSB1A2-I13521 (EcoRI/SpeI)

Product is Bca1014 *internal PstI and NotI sites

ca1014F Forward EcoRI/XbaI for Tnp biobrick
gcaatGAATTCTCTAGAATGATTCTCCGCCAG
ca1014R Reverse SpeI for Tnp biobrick
cctatactagtCGACGGATCAGATCTTGATC

References