Difference between revisions of "Part:BBa K1529300"
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To characterize the function of this <i>rhl(RL)</i> promoter, we constructed a part Prhl(RL)_GFP (<partinfo>BBa_K1529301</partinfo>) by inserting the <i>rhl(RL)</i> promoter, upstream of a GFP coding sequence. | To characterize the function of this <i>rhl(RL)</i> promoter, we constructed a part Prhl(RL)_GFP (<partinfo>BBa_K1529301</partinfo>) by inserting the <i>rhl(RL)</i> promoter, upstream of a GFP coding sequence. | ||
− | [[Image: | + | [[Image:titech2014_parts_rhlpromoter_main_Fig1.jpg|thumb|center|250px|<b>Fig. 1.</b> Our newly designed promoter]] |
By using the reporter cells that contain Prhl(RL)_GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2). | By using the reporter cells that contain Prhl(RL)_GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2). | ||
− | We saw that our new <i>rhl(RL)</i> promoter was actually activated by C4HSL through an induction assay (Fig. | + | We saw that our new <i>rhl(RL)</i> promoter was actually activated by C4HSL through an induction assay (Fig. 2). |
− | [[Image: | + | [[Image:titech2014_parts_GFPassay_main_Fig2.jpg|thumb|center|310px|<b>Fig. 2.</b> Fluorescence intensity detected by flow cytometer]] |
− | + | ||
<br> | <br> |
Revision as of 11:49, 3 October 2014
Prhl(RL)
We newly developed the rhl(RL) promoter (BBa_K1529300) which is activated by C4HSL (Fig. 1).
To characterize the function of this rhl(RL) promoter, we constructed a part Prhl(RL)_GFP (BBa_K1529301) by inserting the rhl(RL) promoter, upstream of a GFP coding sequence.
File:Titech2014 parts rhlpromoter main Fig1.jpg
Fig. 1. Our newly designed promoter
By using the reporter cells that contain Prhl(RL)_GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2). We saw that our new rhl(RL) promoter was actually activated by C4HSL through an induction assay (Fig. 2).
File:Titech2014 parts GFPassay main Fig2.jpg
Fig. 2. Fluorescence intensity detected by flow cytometer
For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]