Difference between revisions of "Part:BBa I20260:Experience"

iGEM12_OUC-China

We improved this part,and submit the improved partBBa_K737068 to the parts registry.

The absolute activity of BioBrick promoters varies across experimental conditions and measurement instruments. Researchers should choose one promoter (BBa_J23101) to serve as an in vivo reference standard for promoter activity. Drew Endy, etc demonstrated that, by measuring the activity of promoters relative to BBa_J23101, we could reduce variation in reported promoter activity due to differences in test conditions and measurement instruments by ~50%. They defined a Relative Promoter Unit (RPU) in order to report promoter characterization data in compatible units and developed a measurement kit so that researchers might more easily adopt RPU as a standard unit for reporting promoter activity. [1] However, unfortunately, there are few data about it and few teams use it as an in vivo control. This year, we detected many RFU (Relative Fluorescence Unit) curves and made it as control group. Our team wants to upload some data about it and hope other teams can use it to decrease the error.

These are RFU curves of K737069, Top10 and I20206 (in PSB4A5).

These are RFU curves of K737052 and I20206 (in PSB4A5).

Reference [1] Kelly, J.r. et al. Measuring the activity of BioBrick promoters using an in vivo reference standard. J. Biol. Eng. 3, 4 (2009).

UNIQ32ad931d19c30bcc-partinfo-00000004-QINU