Difference between revisions of "Part:BBa J100179:Experience"
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Our positive treatment was given the correct sequence after Golden Gate Assembly to produce the red fluorescence. | Our positive treatment was given the correct sequence after Golden Gate Assembly to produce the red fluorescence. | ||
| − | Our expermental treatment was exposed to UV light for 5 seconds, and was then allowed to sit for 30 minutes before extraction. Our negative treatment did not go through Golden Gate Assembly.The cells were resuspended in Luria Broth after scraping them off of plates. Then we pipetted 200 microliters of each treatment into 3 separate wells and ran them through a fluorometer and a spectrophotometer. Then, we divided fluorescence by absorbance to calculate the effectiveness of our promoter. | + | Our expermental treatment was exposed to UV light for 5 seconds, and was then allowed to sit for 30 minutes before extraction. Our negative treatment did not go through Golden Gate Assembly.The cells were resuspended in Luria Broth after scraping them off of plates. Then we pipetted 200 microliters of each treatment into 3 separate wells and ran them through a fluorometer and a spectrophotometer. Alongside the various treatments, we also ran 3 wells containing only the Luria Broth, to act as a benchmark.Then, we divided fluorescence by absorbance to calculate the effectiveness of our promoter. |
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Latest revision as of 13:34, 2 October 2014
This experience page is provided so that any user may enter their experience using this part.
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Our positive treatment was given the correct sequence after Golden Gate Assembly to produce the red fluorescence. Our expermental treatment was exposed to UV light for 5 seconds, and was then allowed to sit for 30 minutes before extraction. Our negative treatment did not go through Golden Gate Assembly.The cells were resuspended in Luria Broth after scraping them off of plates. Then we pipetted 200 microliters of each treatment into 3 separate wells and ran them through a fluorometer and a spectrophotometer. Alongside the various treatments, we also ran 3 wells containing only the Luria Broth, to act as a benchmark.Then, we divided fluorescence by absorbance to calculate the effectiveness of our promoter.
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