Difference between revisions of "Part:BBa J100176:Experience"
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We are testing if triclosan induces the FapR promoter in E.coli cells. This figure shows FapR induction in  E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.<br>
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This figure shows FapR induction in  E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.<br>
 
[[File:PRINTERGRAPH.jpg]]
 
[[File:PRINTERGRAPH.jpg]]
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Purpose: We are testing if triclosan induces the FapR promoter in E.coli cells by fatty acid deprivation.
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Method:

Revision as of 13:20, 2 October 2014

This figure shows FapR induction in E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.
PRINTERGRAPH.jpg

Purpose: We are testing if triclosan induces the FapR promoter in E.coli cells by fatty acid deprivation.

Method: