Difference between revisions of "Part:BBa J100176:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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We are testing if triclosan induces the FapR promoter in E.coli cells. This figure shows FapR induction in E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.<br> | We are testing if triclosan induces the FapR promoter in E.coli cells. This figure shows FapR induction in E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.<br> | ||
Revision as of 12:59, 2 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
We are testing if triclosan induces the FapR promoter in E.coli cells. This figure shows FapR induction in E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.
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