Difference between revisions of "Part:BBa K1412006"
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=='''Usage'''==
 
=='''Usage'''==
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1.We performed a swarming assay to characterize the motility of CheZ-deficient strain CL1 and cells transformed with CheZ-expressing plasmid [https://parts.igem.org/Part:BBa_K1412001  BBa_K1412001 ].
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1.We performed a swarming assay to characterize the motility of ''CheZ''-deficient strain CL1 and cells transformed with ''CheZ''-expressing plasmid [https://parts.igem.org/Part:BBa_K1412001  BBa_K1412001 ].
 
    
 
    
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Our results clearly show that CheZ-deficient mutants show smaller diffusion, while they are rescued by our [https://parts.igem.org/Part:BBa_K1412001  BBa_K1412001] plasmid.
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Our results clearly show that ''CheZ''-deficient mutants show smaller diffusion, while they are rescued by our [https://parts.igem.org/Part:BBa_K1412001  BBa_K1412001] plasmid.
  
  
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2.We also performed a swarming assay to characterize characterize the strength of RBS with CL1 cells. Three strains of CL1 are transformed with CheZ-expressing plasmid of different RBS efficiency ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034 ]:1.0 ; [https://parts.igem.org/Part:BBa_B0032  BBa_B0032 ]:0.3 ; [https://parts.igem.org/Part:BBa_B0033  BBa_B0033 ]:0.01 ).
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2.We also performed a swarming assay to characterize characterize the strength of RBS with CL1 cells. Three strains of CL1 are transformed with ''CheZ''-expressing plasmid of different RBS efficiency ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034 ]:1.0 ; [https://parts.igem.org/Part:BBa_B0032  BBa_B0032 ]:0.3 ; [https://parts.igem.org/Part:BBa_B0033  BBa_B0033 ]:0.01 ).  
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Changing different RBS in the same circuit, we can characterize the efficiency of RBS, since the expression strength of CheZ is positively associated with the efficiency of RBS.
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Changing different RBS in the same circuit, we can characterize the efficiency of RBS, since the expression strength of ''CheZ'' is positively associated with the efficiency of RBS.
  
 
=='''Experimental data''' ==
 
=='''Experimental data''' ==

Revision as of 12:14, 2 October 2014

RBS (0.01) CheZ double terminators -- derivative of BBa_K1412005

This part is a derivative of BBa_K1412005 but weaker than it. It consists of a CheZ coding region and a RBS coding region whose relative efficiency is 0.3. This part does not pose any biological threat. With a promoter of your interest, this device rescues the mobility of CheZ-/- cells. It has been reported that CheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.


Usage

1.We performed a swarming assay to characterize the motility of CheZ-deficient strain CL1 and cells transformed with CheZ-expressing plasmid BBa_K1412001 .

Our results clearly show that CheZ-deficient mutants show smaller diffusion, while they are rescued by our BBa_K1412001 plasmid.


2.We also performed a swarming assay to characterize characterize the strength of RBS with CL1 cells. Three strains of CL1 are transformed with CheZ-expressing plasmid of different RBS efficiency (BBa_B0034 :1.0 ; BBa_B0032 :0.3 ; BBa_B0033 :0.01 ).

Changing different RBS in the same circuit, we can characterize the efficiency of RBS, since the expression strength of CheZ is positively associated with the efficiency of RBS.

Experimental data

IMG 20140825 185642.jpg;


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

[1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ].

[2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia coli: association of protein components. Biochemistry 19: 4633–4639 ].