Difference between revisions of "Part:BBa K1470002"
(Created page with "__NOTOC__ <partinfo>BBa_K1470002 short</partinfo> <br> This protein from <i>Saccharomyces cerevisiae</i> is a positive transcriptional regulator for the gene expression of the g...") |
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| − | This protein from <i>Saccharomyces cerevisiae</i> is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose [1]. <br>It recognizes a 17 base pair sequence (5'-CGGN<sub>11</sub>CCG-3') in the upstream activating sequence (UAS-G) of these genes.<br> | + | This protein from <i>Saccharomyces cerevisiae</i> is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose [1]. <br>It recognizes a 17 base pair sequence (5'-CGGN<sub>11</sub>CCG-3') in the upstream activating sequence (UAS-G) of these genes. [2]<br> |
| − | GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [ | + | GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [3].<br> The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression and detection of the corresponding gene, GFP for example.<br> |
==References== | ==References== | ||
<small> | <small> | ||
| − | TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496–499.<br> | + | [1] TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496–499.<br> |
| − | BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.<br></small> | + | [2] CAMPBELL, R.N., LEVERENTZ, M.L., RYAN, L.A., REECE, R.J.: Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae. Biochem. J. (2008) 414 (177–187). |
| + | [3] BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.<br></small> | ||
Revision as of 12:05, 2 October 2014
Galactose-induced gene 4 DNA binding domain (Gal4DBD)
This protein from Saccharomyces cerevisiae is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose [1].
It recognizes a 17 base pair sequence (5'-CGGN11CCG-3') in the upstream activating sequence (UAS-G) of these genes. [2]
GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [3].
The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression and detection of the corresponding gene, GFP for example.
References
[1] TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496–499.
[2] CAMPBELL, R.N., LEVERENTZ, M.L., RYAN, L.A., REECE, R.J.: Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae. Biochem. J. (2008) 414 (177–187).
[3] BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.