Difference between revisions of "Part:BBa K1463520:Design"

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Made by PCR using E1010 as a template and primers
 
Made by PCR using E1010 as a template and primers
−
XXXX and YYYYY to incorporate prefix at the end and suffix at the beginning.
+
XXXX and YYYYY to incorporate prefix at the 3' end of the rfp gene and the suffix 5' end of rfp.
 +
The  final 'C' of the bar code was omitted, with no effect on rfp function.
  
 
===References===
 
===References===

Latest revision as of 10:38, 2 October 2014


E1010 RFP in reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No specific design considerations other than ensuring that the sequence was in reverse.


Source

Made by PCR using E1010 as a template and primers XXXX and YYYYY to incorporate prefix at the 3' end of the rfp gene and the suffix 5' end of rfp. The final 'C' of the bar code was omitted, with no effect on rfp function.

References