Difference between revisions of "Part:BBa K1402010"
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This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) | This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) | ||
The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: | The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: | ||
| − | (1)repressed by LacI. | + | (1)repressed by LacI. |
| − | (2)induced by lac or IPTG, showing a high expression level. | + | (2)induced by lac or IPTG, showing a high expression level. |
| − | (3)CAP protein will not influence the expression. | + | (3)CAP protein will not influence the expression. |
| − | It is a strong promoter. You can | + | It is a strong promoter. You can only use this part in E.Coli BL21(DE3). |
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Revision as of 08:19, 2 October 2014
Lac Promoter
This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: (1)repressed by LacI. (2)induced by lac or IPTG, showing a high expression level. (3)CAP protein will not influence the expression. It is a strong promoter. You can only use this part in E.Coli BL21(DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]