Difference between revisions of "Part:BBa K1402010"

Revision as of 08:18, 2 October 2014

Lac Promoter

This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: (1)repressed by LacI. (2)induced by lac or IPTG, showing a high expression level. (3)CAP protein will not influence the expression. It is a strong promoter. You can Only use this part in E.Coli BL21(DE3).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1402010 short</partinfo>
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 This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.)
 The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be:
-&#61607;	repressed by LacI
+(1)repressed by LacI.
-&#61607;	induced by lac or IPTG, showing a high expression level.
+(2)induced by lac or IPTG, showing a high expression level.
-&#61607;	CAP protein will not influence the expression.
+(3)CAP protein will not influence the expression.
-Strong promoter. Only E.Coli BL21(DE3) can use this part.
+It is a strong promoter. You can Only use this part in E.Coli BL21(DE3).
 <!-- Add more about the biology of this part here