Difference between revisions of "Part:BBa K1412801"
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BBa_K1412801: <i>Plac-RBS(0.3)-CheZ-TT</i>
  
 
This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of <i>CheZ</i>.
 
This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of <i>CheZ</i>.
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===Usage and Biology===
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=='''Usage'''===
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==='''Usage'''===
 
   
 
   
  
 
When we want to characterize the efficiency of RBS, we usually link the RBS with GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a <i>CheZ </i> gene, ending with a TT terminator(<i>Plac-RBS</i>(changeable)-<i>CheZ-TT</i>). Then transfer this gene circuit into E.coli (<i>CheZ</i> knock out), and coat plates, culuture on semi-solid medium to measure the migration diameter of <i>E.coli</i>.
 
When we want to characterize the efficiency of RBS, we usually link the RBS with GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a <i>CheZ </i> gene, ending with a TT terminator(<i>Plac-RBS</i>(changeable)-<i>CheZ-TT</i>). Then transfer this gene circuit into E.coli (<i>CheZ</i> knock out), and coat plates, culuture on semi-solid medium to measure the migration diameter of <i>E.coli</i>.
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==='''Relevant parts'''===
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<bbpart>BBa_K1412000</bbpart>
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<bbpart>BBa_K1412005</bbpart>
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<bbpart>BBa_K1412006</bbpart>
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<bbpart>BBa_K1412007</bbpart>
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<bbpart>BBa_K1412014</bbpart>
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<bbpart>BBa_K1412614</bbpart>
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<bbpart>BBa_K1412829</bbpart>
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==='''Notes'''===
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Source
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3H:13-P3-3H <bbpart>BBa_R0010<bbpart>
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2J:14-P2-2J <bbpart>Bba_B0032<bbpart>
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18G:14-P1-18G <bbpart>BBa_K629003<bbpart>
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4F:13-P3-4F <bbpart>BBa_B0015<bbpart>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1412801 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1412829 SequenceAndFeatures</partinfo>
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===Protocol===
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genetic link stage
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1.As a skeleton, <i>TT</i> terminator link with <i>CheZ</i>.
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2.Then the skeleton <i>CheZ-TT</i> link to <i>RBS</i>.
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3.Next, link  <i>RBS-CheZ-TT</i> with promoter <i>Plac</i>.
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characterization stage
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1.Transfer the part <i>Plac-RBS-CheZ-TT</i> into E.coli (<i>CheZ</i> knock out), and coat plates, culture for hours to measure the migration diameter of E.coli.
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==='''Reference'''===
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ameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1412801 parameters</partinfo>
 
<partinfo>BBa_K1412801 parameters</partinfo>
 
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Revision as of 03:19, 2 October 2014

Characterize efficiency of RBS with chemotaxis

BBa_K1412801: Plac-RBS(0.3)-CheZ-TT

This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.


Usage

When we want to characterize the efficiency of RBS, we usually link the RBS with GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a CheZ gene, ending with a TT terminator(Plac-RBS(changeable)-CheZ-TT). Then transfer this gene circuit into E.coli (CheZ knock out), and coat plates, culuture on semi-solid medium to measure the migration diameter of E.coli.

Relevant parts

BBa_K1412000

BBa_K1412005

BBa_K1412006

BBa_K1412007

BBa_K1412014

BBa_K1412614

BBa_K1412829



Notes

Source 3H:13-P3-3H BBa_R0010