Difference between revisions of "Part:BBa K1412801"

Revision as of 03:02, 2 October 2014

Characterize efficiency of RBS with chemotaxis

This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of <i>CheZ</i>.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+=='''Usage'''===
+When we want to characterize the efficiency of RBS, we usually link the RBS with GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a <i>CheZ </i> gene, ending with a TT terminator(<i>Plac-RBS</i>(changeable)-<i>CheZ-TT</i>). Then transfer this gene circuit into E.coli (<i>CheZ</i> knock out), and coat plates, culuture on semi-solid medium to measure the migration diameter of <i>E.coli</i>.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>