Difference between revisions of "Part:BBa K1412801"
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<partinfo>BBa_K1412801 short</partinfo>
 
<partinfo>BBa_K1412801 short</partinfo>
  
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<i>E.coli</i> can express CheZ protein showing the chemotaxis of <i>E.coli</i>. When we change different kinds of RBS or promoter in the same circuit, we can characterize the efficiency of RBS and promoter, since the expression strength of CheZ is positively associated with the efficiency of RBS and promoter.
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This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of <i>CheZ</i>.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 03:00, 2 October 2014

Characterize efficiency of RBS with chemotaxis

This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]